diff --git a/tests/files/ubuntu22-calico-all-in-one-upgrade.yml b/tests/files/ubuntu22-calico-all-in-one-upgrade.yml
index ff157b241..0f8a83d25 100644
--- a/tests/files/ubuntu22-calico-all-in-one-upgrade.yml
+++ b/tests/files/ubuntu22-calico-all-in-one-upgrade.yml
@@ -11,6 +11,9 @@ auto_renew_certificates: true
 kube_proxy_mode: iptables
 enable_nodelocaldns: false
 
+# Single node don't need the DNS autoscaler
+enable_dns_autoscaler: false
+
 containerd_registries_mirrors:
   - prefix: docker.io
     mirrors: